Pgadt7-Rec. Plasmid sequence and snapgene enhanced annotations. Use text editor or plasmid mapping software to view sequence.
Add 20μl ddh2o in to. Briefly vortex the tube and.
Add 20Μl Ddh2O In To.
Use text editor or plasmid mapping software to view sequence.
Close The Tube And Incubate For 10 Minutes At Room Temperature.
Download plasmid open in snapgene.
Fig 1) Using The Gateway 1 Lr Clonase Tm Ii Enzyme Mix (Invitrogen).
Images References :
This Recognition Sequence Is Asymmetric, So Ligating Blunt Ends.
Add 20μl ddh2o in to.
A Unique Restriction Site (Smai) Is Shown In Bold.
Use text editor or plasmid mapping software to view sequence.
Function Yeast Hybrid Plasmid Resistance Amp Screen / Strain Dh5Alpha Culture.